This tutorial walks students through a fictional scenario to teach them about cloning. After an overview of the problem, users construct a DNA library through DNA digestion, ligation, and transformation. Finally, the clones are screened through plating, and a method is selected by the student. Students learn the final steps that would be performed to sequence and compare the gene, characterize the enzyme, and learn its structure.
Biology
LESSON MODULE
Sixth grade, Seventh grade, Eighth grade, Ninth grade, Tenth grade, Eleventh grade, Twelfth grade, Undergraduate